PRIM2

Description

The PRIM2 (DNA primase subunit 2) is a protein-coding gene located on chromosome 6.

PRIM2 gene encodes the large subunit of DNA primase, a 58 kDa protein. DNA primase is a heterodimer composed of a small and a large subunit. During DNA replication in eukaryotic cells, primase synthesizes small RNA primers for the Okazaki fragments, which are required for discontinuous DNA replication.

PRIM2 is a regulatory subunit of the DNA primase complex and a component of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex). It plays an essential role in the initiation of DNA synthesis during the S phase of the cell cycle. The DNA polymerase alpha complex, composed of POLA1, POLA2, PRIM1, and PRIM2, is recruited to DNA at the replicative forks via interactions with MCM10 and WDHD1. The primase subunit initiates DNA synthesis by oligomerizing short RNA primers on both leading and lagging strands. These primers are then extended by POLA1 and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strands, respectively. PRIM2, along with PRIM1, is required for the initial di-nucleotide formation, but the extension of the primer depends only on PRIM1. PRIM2 binds RNA:DNA duplex and coordinates the catalytic activities of PRIM1 and POLA2 during the primase-to-polymerase switch.

PRIM2 is also known as PRIM2A, p58.

Associated Diseases

WES Whole Exome Sequencing