NAPEPLD

Description

The NAPEPLD (N-acyl phosphatidylethanolamine phospholipase D) is a protein-coding gene located on chromosome 7.

NAPEPLD, encoded by the NAPEPLD gene, is an enzyme that breaks down N-acyl-phosphatidylethanolamine (NAPE) into N-acylethanolamine (NAE). This process is crucial for converting ordinary lipids into signaling molecules like anandamide and oleoylethanolamine. NAPEPLD's activity was discovered through a series of experiments that culminated in the purification of the enzyme from rat heart tissue. This involved homogenizing the tissue, separating cell membranes, solubilizing the membrane proteins, and using various chromatography steps to isolate the active enzyme. The final product was not a pure protein, but it produced a limited number of bands on SDS-PAGE, and a 46 kDa band correlated with the enzyme activity.

NAPEPLD is a D-type phospholipase that hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce bioactive N-acylethanolamines/fatty acid ethanolamides (NAEs/FAEs) and phosphatidic acid. It cleaves the terminal phosphodiester bond of diacyl- and alkenylacyl-NAPEs, primarily playing a role in the generation of long-chain saturated and monounsaturated NAEs in the brain. NAPEPLD may control NAPE homeostasis in dopaminergic neuron membranes and regulate neuron survival, partly through RAC1 activation. As a regulator of lipid metabolism in the adipose tissue, it mediates the crosstalk between adipocytes, gut microbiota and immune cells to control body temperature and weight. In particular, NAPEPLD regulates energy homeostasis by promoting cold-induced brown or beige adipocyte differentiation program to generate heat from fatty acids and glucose. It has limited D-type phospholipase activity toward N-acyl lyso-NAPEs.

NAPEPLD is also known as C7orf18, FMP30, NAPE-PLD.

Associated Diseases

WES Whole Exome Sequencing