RNA Sequencing

RNA sequencing (RNA-Seq) is a powerful technique that uses next-generation sequencing (NGS) to reveal the presence and quantity of RNA molecules in a biological sample. Let’s explore the details:

  1. Principle of RNA Sequencing:

    • RNA-Seq provides a snapshot of gene expression in a sample, also known as the transcriptome.
    • Unlike gene sequencing (DNA sequencing), which obtains nucleotide sequences of a DNA molecule, RNA-Seq focuses on the order of nucleotide sequences in an RNA molecule.
    • Initially, the Sanger Sequencing technique was used for RNA sequencing, but shortly after the introduction of NGS, high-throughput RNA-Seq became prevalent.

  2. Types of RNA Sequencing:

    • Direct RNA Sequencing:
      • In this method, isolated RNAs are sequenced directly without prior conversion into cDNA.
      • RNAs are inherently unstable compared to DNAs, making them challenging to handle.
      • However, direct sequencing avoids biases introduced during cDNA preparation.

Disclaimer: The information provided here is not exhaustive by any means. Always consult your doctor or other qualified healthcare provider with any questions you may have regarding a medical condition, procedure, or treatment, whether it is a prescription medication, over-the-counter drug, vitamin, supplement, or herbal alternative.