MID2
Description
The MID2 (midline 2) is a protein-coding gene located on chromosome X.
MID2 is a protein encoded by the MID2 gene in humans. It belongs to the tripartite motif (TRIM) family and contains three zinc-binding domains: RING, B-box type 1, and B-box type 2, as well as a coiled-coil region. MID2 localizes to microtubular structures in the cytoplasm. Its primary function is related to cytokinesis, where it ubiquitinates Astrin on K409, promoting Astrin degradation and proper cell division. Depletion of MID2 leads to Astrin accumulation at the midbody, causing cytokinetic arrest, multinucleated cells, and cell death. MID2 interacts with MID1 and LRRK2, a protein often mutated in familial Parkinson's disease.
MID2 is an E3 ubiquitin ligase that promotes microtubule stabilization. It specifically targets LRRK2 for Lys-48-linked polyubiquitination, leading to its localization on microtubules and subsequent degradation via the proteasome in neurons. This ubiquitination process inhibits the kinase activation of LRRK2 by RAB29.
MID2 is also known as FXY2, MRX101, RNF60, TRIM1, XLID101.
