IWS1
Description
The IWS1 (interacts with SUPT6H, CTD assembly factor 1) is a protein-coding gene located on chromosome 2.
IWS1, also known as interacts with Spt6 (IWS1), is a protein encoded by the IWS1 gene in humans. It functions as a transcription elongation factor and was initially discovered in yeast during a search for RNA polymerase II-associated elongation factors. IWS1 directly interacts with RNA polymerase II (RNAPII) and is phosphorylated at casein kinase II (CKII) sites. The human homolog, which physically interacts with protein arginine methyltransferase 5 (PRMT5), is crucial for cell survival. It also recruits a SET2 histone methyltransferase (Huntingtin-interacting protein HYPB, also known as SETD2) to RNAPII during transcription elongation, and is required for H3K36 trimethylation.
IWS1 is a transcription factor that plays a critical role in shaping the RNA polymerase II (RNAPII) elongation complex and regulating the production of mature mRNA transcripts. It acts as an assembly factor, recruiting various factors to the RNAPII elongation complex. IWS1 binds to the transcription elongation factor SUPT6H, which is associated with the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The resulting SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) and histone modifying enzymes (such as SETD2) to ensure proper mRNA splicing, efficient mRNA export, and elongation-coupled H3K36 methylation, a hallmark of active transcription.
IWS1 is also known as -.
