GOT2
Description
The GOT2 (glutamic-oxaloacetic transaminase 2) is a protein-coding gene located on chromosome 16.
GOT2 (Aspartate aminotransferase, mitochondrial) is an enzyme encoded by the GOT2 gene in humans. It is a pyridoxal phosphate-dependent enzyme that exists in mitochondrial form, playing a crucial role in amino acid metabolism, the urea cycle, and the Kreb's cycle. GOT2 is a major participant in the malate-aspartate shuttle, a pathway for transporting reducing equivalents from the cytosol to the mitochondria. It is a homodimeric protein with two identical subunits, exhibiting close homology to its cytoplasmic counterpart, GOT1. GOT2 is involved in cell proliferation, particularly in tumor growth. Structurally, it comprises two identical subunits with overlapping regions. The enzyme's top and sides are composed of helices, while its bottom is formed by beta sheets and extended hairpin loops. The subunit can be divided into four distinct parts: a large domain that binds pyridoxal-P, a small domain, an NH2-terminal arm, and a bridge across the two domains formed by residues 48-75 and 301-358. GOT2's nucleic acid and protein sequences are highly conserved across eukaryotic cells, indicating its ubiquitous nature. Its 5' regulatory regions in genomic DNA resemble those of typical house-keeping genes, lacking a TATA box.
GOT2 is also known as DEE82, KAT4, KATIV, KYAT4, mitAAT.
