CELF2
Description
The CELF2 (CUGBP Elav-like family member 2) is a protein-coding gene located on chromosome 10.
CELF2 is a RNA-binding protein involved in the regulation of various post-transcriptional events, including pre-mRNA alternative splicing, mRNA translation, and stability. It plays a role in mediating exon inclusion and/or exclusion in pre-mRNA, influencing tissue-specific and developmentally regulated alternative splicing. Notably, CELF2 activates exon 5 inclusion of TNNT2 in embryonic skeletal muscle but not in adult muscle. This activation is achieved by antagonizing the repressive effect of PTB. It acts as both an activator and repressor of coregulated exons, promoting the inclusion of the smooth muscle (SM) exon while excluding the non-muscle (NM) exon in actinin pre-mRNAs. Additionally, CELF2 promotes the inclusion of exon 21 and exclusion of exon 5 in the NMDA receptor R1 pre-mRNA. CELF2 is involved in apoB RNA editing activity. In epithelial cells, it increases COX2 mRNA stability and inhibits its translation following radiation injury. It modulates cellular apoptosis by regulating COX2-mediated prostaglandin E2 (PGE2) expression. CELF2 binds to (CUG)n triplet repeats in the 3'-UTR of transcripts like DMPK and to muscle-specific splicing enhancer (MSE) intronic sites flanking the TNNT2 alternative exon 5. It preferentially binds to UG-rich sequences, including UG repeats and UGUU motifs. CELF2 binds to apoB mRNA, specifically to AU-rich sequences upstream of the edited cytidine. It also binds AU-rich sequences in the 3'-UTR of COX2 mRNA and to an intronic RNA element responsible for silencing exon 21 splicing. It binds to (CUG)n repeats and may be a specific regulator of miRNA biogenesis. CELF2 binds to primary microRNA pri-MIR140 and, along with CELF1, negatively regulates its processing into mature miRNA. It interacts with A1CF.
CELF2 is also known as BRUNOL3, CELF-2, CUG-BP2, CUGBP2, DEE97, ETR-3, ETR3, NAPOR.
