AKAP8
Description
The AKAP8 (A-kinase anchoring protein 8) is a protein-coding gene located on chromosome 19.
A-kinase anchor protein 8 is an enzyme that, in humans, is encoded by the AKAP8 gene.
== Function == The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining it to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is located in the nucleus during interphase and is redistributed to distinct locations during mitosis. This protein has a cell cycle-dependent interaction with the RII subunit of PKA.
== Interactions == AKAP8 has been demonstrated to interact with:
Cyclin D3 DDX5, MCM2, MYCBP, and PRKAR2A.
== References ==
== Further reading ==
== External links == AKAP8 human gene location in the UCSC Genome Browser. AKAP8 human gene details in the UCSC Genome Browser.
AKAP8, also known as A-kinase anchor protein 95 kDa, is an anchoring protein that controls the location of cAMP-dependent protein kinase (PKA type II) within cells. It helps to maintain chromosomes in a condensed form during mitosis by anchoring a PKA-signaling complex to them. AKAP8 is also involved in recruiting the condensin complex subunit NCAPD2 to chromosomes, which is essential for chromatin condensation. This function seems to be independent of PKA anchoring. AKAP8 may also help to deliver cyclin D/E to CDK4, promoting cell cycle progression. It is crucial for the G2/M transition and histone deacetylation during mitosis. In mitotic cells, it recruits HDAC3 to chromatin, leading to deacetylation and subsequent phosphorylation of histone H3 at Ser-10. This function may overlap with that of AKAP8L. AKAP8 plays a role in nuclear retention of RPS6KA1 upon ERK activation, promoting cell proliferation. It might also be involved in regulating DNA replication by acting as a scaffold for MCM2. AKAP8 enhances the HMT activity of the KMT2 family MLL4/WBP7 complex, contributing to transcriptional regulation. In a teratocarcinoma cell line, it participates in retinoic acid-mediated induction of developmental genes, likely through H3 Lys-4 methylation. AKAP8 may recruit active CASP3 to the nucleus in apoptotic cells. It could act as a carrier protein for GJA1, transporting it to the nucleus. AKAP8 might have a repressive role in regulating rDNA transcription. It preferentially binds GC-rich DNA in vitro and associates with rRNA chromatin in cells, particularly with the rRNA promoter and transcribed regions. AKAP8 participates in modulating Toll-like receptor signaling. It is required for cAMP-dependent suppression of TNF-alpha in the early stages of LPS-induced macrophage activation, possibly by targeting PKA to NFKB1. It binds to the PKA RII-alpha regulatory subunit PRKAR2A (phosphorylated at Thr-54) during mitosis. AKAP8 interacts with FIGN (via C-terminus), NCAPD2, CCND1, MCM2, RPS6KA1, PDE4A, CCND3, CCNE1, DDX5, CASP3, NFKB1, MYCBP, DPY30, HDAC3, and GJA1.
AKAP8 is also known as AKAP 95, AKAP-8, AKAP-95, AKAP95.
